ANTIBIOTIC SUSCEPTIBILITY TEST

• Main Author: KITTLE, Joseph D
• Format: Patent
• Language: English; French; German
• Published: 21.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A method for determining the susceptibility of a bacteria to an antibiotic, comprising transferring one portion of a sample containing living bacterial cells into a bacterial growth medium to create a control sample; transferring another portion of the sample into a bacterial growth medium to which a predetermined amount of antibiotic or predetermined amount of a library of antibiotics has been added to create a test sample; adding an alkyne-modified non-canonical amino acid to both the control sample and test sample during bacterial growth, wherein the alkyne-modified non-canonical amino acid incorporates into surface proteins, internal proteins, or both; reacting the alkyne-containing proteins with an azide-modified detection molecule using click-chemistry to label the cells; detecting the labeled cells using a method that generates a detectable signal; and comparing the signal generated by the control sample to the signal generated by the test sample, wherein a decrease in detectable signal between the control sample and the test sample is indicative of susceptibility of the living bacteria to the predetermined antibiotic or predetermined library of antibiotics.