A PROCESS TO PRODUCE A PHARMACEUTICAL COMPOSITION

The present invention relates to a cell culture process for producing a monoclonal antibody composition, the process comprising culturing the mammalian cells at a pH range of about 6.6 to about 7.5, performing a temperature shift from a first culture temperature to a second culture temperature, supp...

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Bibliographic Details
Main Authors PUDIPEDDI, Akhila, KANNAN, Sneha, BANDYOPADHYAY, Suman, SWAYAMSIDDH, Saswat, PARUPUDI, L C Pavani
Format Patent
LanguageEnglish
French
German
Published 26.06.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a cell culture process for producing a monoclonal antibody composition, the process comprising culturing the mammalian cells at a pH range of about 6.6 to about 7.5, performing a temperature shift from a first culture temperature to a second culture temperature, supplementation of galactose in the cell culture, thereby obtaining an antibody composition comprising galactosylated glycoform and/or G0F glycoform. Further, the cell culture process disclosed in the present invention obtains an antibody composition comprising galactosylated glycoform wherein the percentage of galactosylated glycoform decreases with the decrease in the difference between the first temperature and the second temperature.
Bibliography:Application Number: EP20220858045