DIRECT DETECTION OF SINGLE MOLECULES ON MICROPARTICLES

The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provid...

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Bibliographic Details
Main Authors PROSTKO, John, HAACK, Richard, TETIN, Sergey Y, POPE, Mark R, MACDONALD, Patrick J, QIU, Xiaoxing, RUAN, Qiaoqiao, SWIFT, Kerry M, CALFIN, Brenda, LIN, Zhen, BOGDAN, M. Felicia
Format Patent
LanguageEnglish
French
German
Published 24.07.2024
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.
Bibliography:Application Number: EP20240167359