RECOMBINANT YEAST CELL

A recombinant yeast cell functionally expressing: a) a nucleic acid sequence encoding an enzyme having NADH-dependent nitrate reductase activity and/or a nucleic acid sequence encoding an enzyme having NADH-dependent nitrite reductase activity; and b) a nucleic acid sequence encoding a protein havin...

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Main Authors VUGT- VAN LUTZ, Ingrid Maria, JANSEN, Mickel Leonardus August, DE JONG, René Marcel, DE BRUIJN, Hans Marinus Charles Johannes, VAN RIJ, Evert Tjeerd, BUREMAN, Phillip E, ROSSELL-ARAGORT, Sergio Luis, SCHMITZ, Jozef Petrus Johannes
Format Patent
LanguageEnglish
French
German
Published 22.05.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A recombinant yeast cell functionally expressing: a) a nucleic acid sequence encoding an enzyme having NADH-dependent nitrate reductase activity and/or a nucleic acid sequence encoding an enzyme having NADH-dependent nitrite reductase activity; and b) a nucleic acid sequence encoding a protein having transketolase activity (EC 2.2.1.1),wherein the expression of the nucleic acid sequence encoding the protein having transketolase activity is under control of a promoter (the "TKL promoter"), which TKL promoter has an anaerobic/aerobic expression ratio for the transketolase of 2 or more.
Bibliography:Application Number: EP20220747315