A METHOD OF INCREASING THE PRODUCTIVITY OF EUCARYOTIC CELLS IN THE PRODUCTION OF RECOMBINANT FVIII

A method of increasing the productivity, in particular cell-specific productivity, of recombinant factor VIII (rFVIII) produced in an eukaryotic cell suspension during culturing of said eucaryotic cell suspension in a culturing medium containing not more than 500 µM CaCl2, at least a non-ionic deter...

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Bibliographic Details
Main Authors Aizawa, Peter, Agerkvist, Irène
Format Patent
LanguageEnglish
French
German
Published 17.04.2024
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:A method of increasing the productivity, in particular cell-specific productivity, of recombinant factor VIII (rFVIII) produced in an eukaryotic cell suspension during culturing of said eucaryotic cell suspension in a culturing medium containing not more than 500 µM CaCl2, at least a non-ionic detergent and other nutrient components needed for the cells to grow and produce rFVIII, characterized in that the said cell suspension is cultured under conditions inducing a shear stress by mechanical means to the eucaryotic cell suspension by adding power density of at least 3 W/m3.
Bibliography:Application Number: EP20230211876