PURIFICATION OF FVIII FROM PLASMA USING SILICON OXIDE ADSORPTION

Efficient methods for capture and removal of fibrinogen from blood plasma fractions, especially cryoprecipitate, and Fraction II+III providing high yields of blood coagulation Factor VIII are disclosed. According to this disclosure, there is provided a method of separating plasma cryoprecipitate or...

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Bibliographic Details
Main Authors DER-SARKISSIAN, Ani, GAVIT, Patrick D, ZAYDENBERG, Alexander, HASSOUNA, Hany Anwar, BADDOUR, Yasser, YU, Joshua Elliot, VINCENT, Emily
Format Patent
LanguageEnglish
French
German
Published 13.09.2023
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
TRANSPORTING
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Summary:Efficient methods for capture and removal of fibrinogen from blood plasma fractions, especially cryoprecipitate, and Fraction II+III providing high yields of blood coagulation Factor VIII are disclosed. According to this disclosure, there is provided a method of separating plasma cryoprecipitate or Fraction II+III comprising a blood coagulation factor and fibrinogen into a first fraction comprising the blood coagulation factor and a second fraction containing the fibrinogen, the method comprising: (a) contacting he plasma cryoprecipitate with solid SiO2 or Al(OH)3, thereby adsorbing the fibrinogen onto the solid SiO2 or Al(OH)3; and (b) separating the fibrinogen adsorbed onto the solid SiO2 or Al(OH)3 from the blood factor, thereby forming the first fraction and the second fraction.
Bibliography:Application Number: EP20210852007