DNA-CONTAINING POLYNUCLEOTIDES AND GUIDES FOR CRISPR TYPE V SYSTEMS, AND METHODS OF MAKING AND USING THE SAME

• Main Author: DONOHOUE, Paul, Daniel
• Format: Patent
• Language: English; French; German
• Published: 23.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure provides guides for use in Type V CRISPR systems wherein such guides contain ribonucleotide bases and at least one deoxyribonucleotide base. CRISPR Casl2 guides that contain at least one deoxyribonucleotide base, as well as nucleoprotein complexes of Type V CRISPR-Casl2 protei...