DNA-CONTAINING POLYNUCLEOTIDES AND GUIDES FOR CRISPR TYPE V SYSTEMS, AND METHODS OF MAKING AND USING THE SAME

The present disclosure provides guides for use in Type V CRISPR systems wherein such guides contain ribonucleotide bases and at least one deoxyribonucleotide base. CRISPR Casl2 guides that contain at least one deoxyribonucleotide base, as well as nucleoprotein complexes of Type V CRISPR-Casl2 protei...

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Bibliographic Details
Main Author DONOHOUE, Paul, Daniel
Format Patent
LanguageEnglish
French
German
Published 23.08.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present disclosure provides guides for use in Type V CRISPR systems wherein such guides contain ribonucleotide bases and at least one deoxyribonucleotide base. CRISPR Casl2 guides that contain at least one deoxyribonucleotide base, as well as nucleoprotein complexes of Type V CRISPR-Casl2 proteins and such guides, are also described. Also disclosed are methods for making and using the deoxyribonucleotide-containing polynucleotides and guides, and for making and using the nucleoprotein complexes. Also disclosed are methods for engineering cells using Cast 2 chRDNA guide/nucl eoprotein complexes to produce CAR-expressing cells; and the use of such CAR -expressing cells in adaptive cell therapy.
Bibliography:Application Number: EP20210816566