METHODS OF ASSAYING A BIOLOGICAL CELL

Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can enco...

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Bibliographic Details
Main Authors DAILEY, Katrine Elise, KIM, John Junyeon, SHANSAB, Maryam, MAST, Joshua David, KUBIT, Matthew Asuka, KUBATUR, Shruthi Sreedhar, ZHOU, Hongye, HU, Zhen, TENNEY, John A, PARK, Minha, PAI, Vincent Haw Tien, HIGA, Adrienne T, NG, Preston Lock, BRIGGS, Jason C, ELLEFSON, Arlvin Louis, TUNG, Po-Yuan, INGRAM, Patrick N, MCEWEN, Jason M, OLSON, Alexander Gerald
Format Patent
LanguageEnglish
French
German
Published 05.07.2023
Subjects
HUMAN NECESSITIES
HYGIENE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEDICAL OR VETERINARY SCIENCE
PHYSICS
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
TESTING
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Summary:Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.
Bibliography:Application Number: EP20210865155