METHOD AND KIT FOR THE DETECTION OF THE SARS-COV-2 VIRUS BASED ON A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP) AND OPTICAL AND/OR ELECTROCHEMICAL DETECTION

• Main Authors: ELETXIGERRA HERNANDEZ, Unai, GOÑI DE CERIO, Felipe, OLABARRIA DE PABLO, Garbiñe, DIEZ AHEDO, Ruth, RIVAS MACHO, Ana, MERINO ALVAREZ, Santos
• Format: Patent
• Language: English; French; German
• Published: 28.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention provides a method for the detection and/or diagnosis of the SARS-CoV-2 virus (COVID-19), in an isolated biological sample, by means of reverse transcription loop-mediated isothermal amplification (RT-LAMP), comprising the amplification of specific regions of the SARS-CoV-2 virus, using a set of specially designed RT-LAMP primers, and wherein the detection of the amplification products is carried out by means of an optical and/or electrochemical method. The present invention also relates to the set of specially designed RT-LAMP primers, to a reading reagent and to a kit for specifically detecting the amplified sequences of the SARS-CoV-2 virus comprising the set of specially designed RT-LAMP primers according to the present invention.