ENGINEERED CAS9-NUCLEASES AND METHOD OF USE THEREOF

• Main Authors: YUEN, Tsz Lo, WONG, Siu Lun
• Format: Patent
• Language: English; French; German
• Published: 21.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Disclosed are SaCas9 protein variants, constructs encoding such variants, compositions comprising such variants and constructs, and methods of using the variants, constructs, and compositions. In some forms, the disclosed variants comprise the mutation Y239H and do not comprise the mutation R245A. A...