HUMANIZED ANTIBODY SPECIFIC TO BACILLUS ANTHRACIS PROTECTIVE ANTIGEN AND PREPARATION METHOD THEREOF

• Main Authors: YU, Chi-Ho, CHOI, Jun-Young, KIM, Chang-Hwan, SHIN, Young-Kee, JEONG, Seong-Tae, JUN, Seung-Chul, JOE, Hae-Eun, HUR, Gyeung-Haeng, SONG, Dong-Hyun, JEONG, Hyun-Ho, KIM, Na-Young
• Format: Patent
• Language: English; French; German
• Published: 07.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a humanized antibody specific to a Bacillus anthracis protective antigen and a preparation method thereof. An antibody library is created by obtaining a Bacillus anthracis protective antigen antibody of a monkey from immune cells obtained by immunizing a Bacillus anthracis protective antigen, an antibody specific to the Bacillus anthracis protective antigen is selected from the created antibody library, and, on the basis of the antibody, a humanized antibody specific to the Bacillus anthracis protective antigen is finally selected by creating a humanized antibody library against a Bacillus anthracis protective antigen. The selected humanized antibody specific to the Bacillus anthracis protective antigen exhibits very high Bacillus anthracis protective antigen affinity and toxin neutralization capacity and has a neutralizing effect in the body before and after exposure to Bacillus anthracis, and thus can be used as a therapeutic composition for treating or preventing an anthrax.