SINGLE DOMAIN VHH ANTIBODIES AGAINST SARS-COV-2 VIRUS

One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps:(a) binding the antigen of interest to beads made...

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Main Authors LÓPEZ, Natalia, SALINAS, Constanza, PINTO, Teresa, CHEUQUEMILLA, Yorka, UBERTI, Benjamín, JARA, Ronald, MULLER, Ananda, VALENZUELA, Guillermo, HIMELREICHS, Johanna, CUEVAS, Alexei, ROJAS, Alejandro, MIRANDA, Zaray
Format Patent
LanguageEnglish
French
German
Published 24.07.2024
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Summary:One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps:(a) binding the antigen of interest to beads made of protein-binding polymers(b) incubating the microorganisms of the expression library with the pearls of stage (a),(c) placing the beads of step (b) in a tube with an inert medium with a density greater than or equal to 1 g/mL and centrifuge for 45 s to 2 minutes at a rate between 150 and 250 g;d) discarding the upper fraction and supernatant with the free microorganisms, and obtain the beads with the linked microorganisms, which correspond to those that express HHV that recognizes the antigen.
Bibliography:Application Number: EP20210804589