P450 BM3 MONOOXYGENASE VARIANTS FOR C19-HYDROXYLATION OF STEROIDS

The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the productio...

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Main Authors KRETSCHMANN, Nils, LINNHOFF, Ruben, BULUT, Dalia, SKALDEN, Lilly, ZORN, Ludwig, PILLING, Jens, HELFRICH, Petra, COCO, Wayne, KENSCH, Oliver, RICHTER, Florian, BERENDES, Frank, TRENNER, Sabine, LUDWIG, Marcus, THEDE, Kai, BURMEISTER, Jens, WAGNER, Jakob
Format Patent
LanguageEnglish
French
German
Published 14.12.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the production of estrone and estradiol. The invention further relates to nucleotide sequences, constructs and vectors for the expression of these P450-BM3 variants
Bibliography:Application Number: EP20210703395