METHOD FOR SINGLE-CHANNEL FREE-FLOW ELECTROPHORESIS WITH SEQUENTIAL PH ADJUSTMENT
Embodiments described herein relate to single-channel free-flow electrophoresis devices or apparatuses, and methods for separating and collecting analytes of interest from a sample by sequentially adjusting the pH value of the electrolyte buffers and separating the analyte of interest according to t...
Saved in:
Main Authors | , , |
---|---|
Format | Patent |
Language | English French German |
Published |
17.08.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Embodiments described herein relate to single-channel free-flow electrophoresis devices or apparatuses, and methods for separating and collecting analytes of interest from a sample by sequentially adjusting the pH value of the electrolyte buffers and separating the analyte of interest according to the corresponding isoelectric points of the analyte of interest. The method includes flowing a sample through a single center channel, applying an electric field perpendicular to a flow direction of the sample via an anolyte channel and a catholyte channel that are parallel to the center channel, and then collecting a fraction of the analyte of interest in accordance with their respective isoelectric points. |
---|---|
Bibliography: | Application Number: EP20200874350 |