METHOD FOR SINGLE-CHANNEL FREE-FLOW ELECTROPHORESIS WITH SEQUENTIAL PH ADJUSTMENT

• Main Authors: XU, Hui, ROACH, David John, YANG, Tom Weisan
• Format: Patent
• Language: English; French; German
• Published: 17.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER,MECHANICAL EFFICIENCY, OR FLUID PRESSURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SEPARATION; SPIRITS; TESTING; TRANSPORTING; VINEGAR; WINE

Embodiments described herein relate to single-channel free-flow electrophoresis devices or apparatuses, and methods for separating and collecting analytes of interest from a sample by sequentially adjusting the pH value of the electrolyte buffers and separating the analyte of interest according to the corresponding isoelectric points of the analyte of interest. The method includes flowing a sample through a single center channel, applying an electric field perpendicular to a flow direction of the sample via an anolyte channel and a catholyte channel that are parallel to the center channel, and then collecting a fraction of the analyte of interest in accordance with their respective isoelectric points.