SUPER-RESOLUTION IMAGING SYSTEM AND METHOD, BIOLOGICAL SAMPLE IDENTIFICATION SYSTEM AND METHOD, NUCLEIC ACID SEQUENCING IMAGING SYSTEM AND METHOD, AND NUCLEIC ACID IDENTIFICATION SYSTEM AND METHOD

• Main Authors: WEI, Dong, NI, Ming, NI, Jielei, SU, Zeyu, ZHOU, Fan, LIANG, Yuanqing, SHEN, Mengzhe, XU, Xun, JI, Ke, LI, Mei
• Format: Patent
• Language: English; French; German
• Published: 06.07.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) comprises an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) comprises an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light. The structured light generation and modulation device (11, 11a) comprises a structured light control device (113, 113a). The structured light control device (113, 113a) controls the structured light generation and modulation device (11, 11a) to change the phase of the structured light output by the structured light generation and modulation device and the direction of an illumination pattern of the structured light projected on the biological sample. The imaging system (B) photographs images of excited light excited by excitation light of at least a wavelength in at least an imaging field of view, in conjunction with the change in the phase of the structured light and/or the direction of the illumination pattern, thus improving the imaging efficiency.