PROTEIN HAVING ACTIVITY OF CATALYZING ALPHA-1,6-GLUCOSYL TRANSFER REACTION

• Main Authors: AIZAWA, Kenta, KANAI, Kenta, TAKECHI, Noriaki, KANAI, Mioka, IIZUKA, Takahisa
• Format: Patent
• Language: English; French; German
• Published: 23.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FODDER; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

An object of the present invention is to provide a protein that catalyzes an α-1,6-glucosyl transfer reaction and can efficiently produce an α-1,6-glucan, an enzyme preparation for producing an α-1,6-glucan, which comprise said protein as an active ingredient, and a method for producing an α-1,6-glucan using the enzyme preparation. The present invention provides a protein having an activity for catalyzing an α-1,6-glucosyl transfer reaction, which is any of the proteins (a) to (c) mentioned below: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 3; (b) a protein consisting of an amino acid sequence having an amino acid sequence identity of 90% or higher to the amino acid sequence of SEQ ID NO: 3; and (c) a protein consisting of an amino acid sequence in which one or several amino acid(s) have been substituted, inserted, deleted and/or added in the amino acid sequence of SEQ ID NO: 3. The present invention provides an enzyme preparation for use in production of an α-1,6-glucan from an oligosaccharide and/or polysaccharide having an α-1,4-glucosidic linkage and/or an α-1,6-glucosidic linkage, which contains the aforementioned protein. The present invention provides a method for producing an α-1,6-glucan, which comprises the reaction step of allowing the aforementioned enzyme preparation to act on an oligosaccharide and/or polysaccharide having an α-1,4-glucosidic linkage and/or an α-1,6-glucosidic linkage to obtain an α-1,6-glucan.