RECOMBINANT SPIDER SILK EXTRUDATE FORMULATIONS

• Main Authors: KITTLESON, Joshua, Tyler, RICE, Gregory, Wilson, WRAY, Lindsay, BOULET-AUDET, Maxime, GUERETTE, Paul, Andre, EL-DIFRAWY, Nour, Eldien
• Format: Patent
• Language: English; French; German
• Published: 16.08.2023
• Subjects: APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBONFILAMENTS; CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS,THREADS, FIBRES, BRISTLES OR RIBBONS; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; NATURAL OR ARTIFICIAL THREADS OR FIBRES; ORGANIC MACROMOLECULAR COMPOUNDS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; SPINNING; TEXTILES; THEIR PREPARATION OR CHEMICAL WORKING-UP

Disclosed herein are recombinant spider silk compositions formed from a silk-based extrudate, such as stable films that adsorb to the skin, and methods for making these compositions. In some embodiments, provided herein is a method of making a silk-based emulsion, comprising: mixing a composition comprising a recombinant spider silk polypeptide powder and glycerol by applying pressure and shear force to the composition, thereby transforming the composition to an extrudate; suspending at least a portion of said extrudate in an aqueous solvent to form an aqueous extrudate suspension; and mixing said aqueous extrudate suspension into an emulsion to form said silk-based emulsion.