COMPOSITION FOR PROCESSING POLYNUCLEOTIDES

• Main Authors: GOPALAN, Vijay Kumar Sreenivasa, HARADA, Josephine, LENJI, Mohammad Rahimi, PFEIFFER, Katherine, MCDERMOTT, Geoffrey, BENT, Zachary, TERRY, Jessica Michele, BELGRADER, Phillip, STUELPNAGEL, John R, HINDSON, Christopher, MIKKELSEN, Tarjei Sigurd, PRICE, Andrew D, O'KEEFFE, Christopher Joachim, SAXONOV, Serge, RYVKIN, Paul, MEER, Elliott
• Format: Patent
• Language: English; French; German
• Published: 31.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for tagging analytes for characterization.