PREPARATION OF A SAMPLE FOR HIGH PRESSURE FREEZING

The present invention relates to a device (10) for preparing a microscopic sample (1) for a high-pressure freezing process in which the sample (1) is provided using an arrangement comprising a middle plate (33) of a high-pressure freezing cartridge (30) and an incubation chamber (100, 200), the midd...

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Bibliographic Details
Main Authors Wogritsch, Rainer, Wurzinger, Paul
Format Patent
LanguageEnglish
French
German
Published 09.03.2022
Subjects
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Summary:The present invention relates to a device (10) for preparing a microscopic sample (1) for a high-pressure freezing process in which the sample (1) is provided using an arrangement comprising a middle plate (33) of a high-pressure freezing cartridge (30) and an incubation chamber (100, 200), the middle plate (33) being attached to the incubation chamber (100, 200) and being detachable from the incubation chamber (100, 200) by effecting a relative movement between the middle plate (33) and the incubation chamber (100, 200), and the sample (1) being provided on an enclosing element (37) which is fitted into an opening (36) of the middle plate (33), wherein the device (10) comprises an engagement structure (11, 21) adapted to engage with the middle plate (33) and to restrict a movement of the middle plate (33) while the incubation chamber (100, 200) is moved, such as to effect said relative movement between the middle plate (33) and the incubation chamber (100, 200) and to thereby detach the middle plate (33) from the incubation chamber (100, 200). Also, an incubation chamber (100, 200), a middle plate (33) of a high-pressure freezing cartridge (30), an arrangement comprising these components, and a corresponding method are part of the present invention.
Bibliography:Application Number: EP20200194615