METHOD FOR SEPARATION OF ANTIBODIES OR ANTIBODY FRAGMENTS BEING DEVOID OF AN FC REGION CAPABLE OF BINDING TO PROTEIN A

The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation...

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Bibliographic Details
Main Authors ANDER, Mats, BJÖRKMAN, Tomas, PALMGREN, Ronnie, BRINK, Charlotte, RODRIGO, Gustav
Format Patent
LanguageEnglish
French
German
Published 09.03.2022
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation resin having covalently coupled ligands, wherein the ligands comprise a polypeptide as defined by SEQ ID NO 1 and wherein the antibodies or antibody fragments bind to the separation resin; c) optionally washing the separation resin with a washing liquid; d) eluting the antibodies or antibody fragments from the separation resin with an elution liquid and recovering the antibodies or antibody fragments.
Bibliography:Application Number: EP20200723090