RNA-APTAMER-SENSORS

The present invention relates to a method for optimizing the production of a RNA sequence of interest in a cell, comprising the steps of a) introducing into a plurality of cells a vector capable of expressing a heterologous RNA of interest, wherein said RNA is tagged with a RNA tag comprising an apt...

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Bibliographic Details
Main Authors Flachbart, Lion, Mahr, Regina, Schaumann, Georg
Format Patent
LanguageEnglish
French
German
Published 02.03.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a method for optimizing the production of a RNA sequence of interest in a cell, comprising the steps of a) introducing into a plurality of cells a vector capable of expressing a heterologous RNA of interest, wherein said RNA is tagged with a RNA tag comprising an aptamer capable of stabilizing a fluorophore and a scaffold capable of stabilizing the aptamer; b) culturing the cells in a culture medium under conditions that allow expression of the heterologous RNA of interest; c) adding said fluorophore to the culture medium; and d) identifying those cells that show the highest intensity of fluorescence and therefore a high expression of the RNA of interest.
Bibliography:Application Number: EP20200193971