MANUFACTURING METHOD FOR SUBSTRATE ON WHICH NERVE CELLS ARE ARRANGED

• Main Authors: MATSUMOTO, Takahiko, IWASHITA, Natsuko, YAMAZAKI, Takehiro, NONOYAMA, Yusuke, SAMESHIMA, Tatsuya, YAGINUMA, Hidekazu, YUMOTO, Masayuki, SATOH, Naoki
• Format: Patent
• Language: English; French; German
• Published: 29.09.2021
• Subjects: ADDITIVE MANUFACTURING TECHNOLOGY; ADDITIVE MANUFACTURING, i.e. MANUFACTURING OFTHREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVEAGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING,STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING; APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

An object of the present invention is to provide a technique for precisely arranging nerve cells on a substrate while suppressing the migration of nerve cells.A manufacturing method for a substrate on which nerve cells are arranged is provided, the method including a step of arranging, on a substrate, a plurality of liquid droplets containing nerve cells by an inkjet method to form one or a plurality of liquid pools, the substrate having a region in which a cell adhesive material is arranged and a region in which a cell non-adhesive material is arranged; and a step of incubating the liquid pool until the nerve cells sediment and temporarily adhere onto the substrate to form a cell aggregate. The diameter per one liquid pool is 500 µm or less, and the density of nerve cells per one liquid pool is 105 cells/cm2 or more.