COMPOSITIONS AND METHODS FOR DIGITAL POLYMERASE CHAIN REACTION
In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises distinguishing between a true mutation in a polynucleotide and a random error introduced during an amplification step. In some embodiments, the...
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Main Authors | , , , |
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Format | Patent |
Language | English French German |
Published |
30.03.2022
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Subjects | |
Online Access | Get full text |
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Summary: | In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises distinguishing between a true mutation in a polynucleotide and a random error introduced during an amplification step. In some embodiments, the methods reduce the number of false positives reported by a digital PCR assay. In some embodiments, the methods improve the accuracy of a digital PCR assay. |
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Bibliography: | Application Number: EP20190831329 |