DNA ENCAPSULED IN SILICON DIOXIDE ON MAGNETIC NANOPARTICLES

• Main Authors: STRAUSS, Karin, KOHLL, Alexander Xavier Christof, CHEN, Weida, GRASS, Robert, NGUYEN, Bichlien Hoang
• Format: Patent
• Language: English; French; German
• Published: 24.01.2024
• Subjects: BASIC ELECTRIC ELEMENTS; BEER; BIOCHEMISTRY; CALCULATING; CASTING; CHEMISTRY; COMPOSITIONS THEREOF; COMPUTER SYSTEMS BASED ON SPECIFIC COMPUTATIONAL MODELS; COMPUTING; COUNTING; CULTURE MEDIA; ELECTRICITY; ENZYMOLOGY; INDUCTANCES; MAGNETS; MAKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NANOTECHNOLOGY; PERFORMING OPERATIONS; PHYSICS; POWDER METALLURGY; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; SPIRITS; TRANSFORMERS; TRANSPORTING; VINEGAR; WINE; WORKING METALLIC POWDER

A data storage medium is disclosed comprising a substrate covered with alternating layers of a polycationic molecule and artificially synthesized DNA molecules encoding digital information. The magnetic substrate may be a metallic nanoparticle formed from a metal such as iron or cobalt. The polycationic molecule may be polyethyleneimine (PEI). The DNA is protected from degradation by encapsulation in silica. A process for stably storing DNA is also disclosed. Stored DNA may be freed from the silica for sequencing or other analysis by washing the silica-coated DNA with a buffered hydrogen fluoride solution. Storage densities of more than 7% DNA by weight are achieved on nanoparticles.