COMPOSITIONS, DEVICES, SYSTEMS, AND METHODS FOR USING A NANOPORE

• Main Authors: CHEN, Roger Jinteh, Arrigo, DUNBAR, William, B, DEAMER, David, W, BRANTON, Daniel, ABU-SHUMAYS, Robin, HURT, Nicholas, ENDO, Seiko L, LIEBERMAN, KATE, AKESON, Mark, A, WILSON, Noah, A
• Format: Patent
• Language: English; French; German
• Published: 03.01.2024
• Subjects: APPARATUS THEREFOR; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; ELECTROLYTIC OR ELECTROPHORETIC PROCESSES FOR THE PRODUCTIONOF COMPOUNDS OR NON-METALS; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (∼ >50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.