COMPOSITIONS AND METHODS FOR EX VIVO EXPANSION OF HUMAN HEMATOPOIETIC STEM/PROGENITOR CELLS

• Main Authors: CHAI, Li, BRADNER, James Elliot, TATETSU, Hiro, TENEN, Daniel Geoffrey, FEDERATION, Alexander
• Format: Patent
• Language: English; French; German
• Published: 17.02.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TRAPPING; VINEGAR; WINE

Described herein are methods of genetically modifying a quantity of expanded hematopoietic cells, comprising providing a quantity of hematopoietic cells; culturing the quantity of hematopoietic cells in the presence of at least one histone deacetylase inhibitor (HDACi) and at least one growth factor to expand the hematopoietic cells; and contacting the expanded hematopoietic cells with a nucleic acid sequence. The nucleic acid sequence modifies the quantity of expanded hematopoietic cells. Also described are compositions comprising the quantity of expanded hematopoietic cells produced.