DEVICE AND METHOD FOR NUCLEIC ACID MANIPULATION

• Main Authors: KUNG, Li-yun, A, JACOBSON, Joseph, LEAKE, Devin
• Format: Patent
• Language: English; French; German
• Published: 26.05.2021
• Subjects: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; METALLURGY; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; THEIR RELEVANT APPARATUS; TRANSPORTING

Devices and methods are provided for selectively expelling and/or transferring nucleic acids. In one aspect, the device includes a component (e.g., a piezoelectric or an acoustic component) configured to align with one or more features on a solid support, such that when in use, the component (e.g., the piezoelectric or acoustic component) generates a mechanical force to selectively expel and/or transfer one or more volumes of nucleic acid from the solid support. The solid support can include a plurality of discrete features, each feature having a volume (e.g., droplet) of nucleic acid thereon. A power source can be included to provide an electric current to the component (e.g., the piezoelectric or acoustic component, if present) to generate mechanical force. The device can be used for nucleic acid singulation during and/or after assembly.