RECOMBINATION ACTIVATING GENE (RAG) INDUCED V(D)J GENE TARGETING
The present invention relates to methods for targeted insertion of at least one nucleic acid sequence/s of interest into a target genomic locus of a mammalian cell. More specifically, the methods of the invention are based on using nucleic acid cassettes comprising the nucleic acid sequence/s of int...
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Main Authors | , , , , , |
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Format | Patent |
Language | English French German |
Published |
26.02.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention relates to methods for targeted insertion of at least one nucleic acid sequence/s of interest into a target genomic locus of a mammalian cell. More specifically, the methods of the invention are based on using nucleic acid cassettes comprising the nucleic acid sequence/s of interest and at least one recognition signal sequence (RSS), for insertion of the nucleic acid sequence of interest into the target genomic locus that is mediated by RAG-catalyzed recombination. The invention further provides cassettes, vectors and vehicles and cells comprising said cassettes, compositions and uses thereof in immunotherapy. |
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Bibliography: | Application Number: EP20180786980 |