MEANS AND METHODS FOR VISUALIZATION OF TISSUE STRUCTURES

• Main Authors: Gretz, Norbert, Weinfurter, Stefanie, Khan, Arif ul Maula, Huang, Jiaguo, Rudolf, Rüdiger, Brenna, Cinzia
• Format: Patent
• Language: English; French; German
• Published: 27.03.2024
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; MEASURING; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PHYSICS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP

The present invention relates to a chemical compound comprising (i) a polycationic polymer, coupled to (ii) a dye. The present invention further relates to a method for visualizing a glycosamine-containing structure in a biological sample comprising a) contacting an inner lumen of said biological sample with a dye-conjugated polycationic polymer, preferably with the chemical compound according to the present invention; b) tissue-clearing said biological sample; and, thereby, c) visualizing an internal glycosamine-containing structure in said biological sample. The present invention also relates to a method for determining the number and/or size of glomeruli in a kidney or a sample thereof making use of the method for visualizing a glycosamine-containing structure; and also relates to kits and uses related to said chemical compounds and said methods.