METHOD OF CULTIVATION OF HUMAN SALIVARY GLAND CELLS

The present invention is intended to increase the number of human salivary gland cell passages, maintain their undifferential condition and high proliferative potential during cultivation. The culture method of human salivary gland epithelial progenitor cells comprising: (a) obtaining human salivary...

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Main Authors PETRAKOVA, Olga Sergeevna, VASILIEV, Andrey Valentinovich, BORISOV, Mikhail Aleksandrovich, VOROTELYAK, Ekaterina Andreevna, GVAZAVA, Inessa Givievna, ROGOVAYA, Olga Sergeevna
Format Patent
LanguageEnglish
French
German
Published 14.08.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention is intended to increase the number of human salivary gland cell passages, maintain their undifferential condition and high proliferative potential during cultivation. The culture method of human salivary gland epithelial progenitor cells comprising: (a) obtaining human salivary gland epithelial progenitor cells from recipient organism; (b) cell transfer into PCT Epidermal Keratinocyte Medium and cultivation in culture flasks ensuring cell adhesion at 37° C. with addition of 5% CO2 and medium change every 2-4 days until monolayer is reached; (c) cell passage at 1:3-1:5 dilution ratio, including cell removal from the culture flask surface using EDTA trypsin solution and transfer into the new culture flasks; (d) further cell cultivation as defined in claim (b) with in-process medium change every 2-4 days and passaging until monolayer is reached, as defined in claim (c) at a maximum dilution ratio of 1:2-1:3, where the first medium change after each passage shall be provided within 8-24 hours.
Bibliography:Application Number: EP20170858803