TRANSPOSITION INTO NATIVE CHROMATIN FOR ANALYSIS OFCHROMATIN
Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagge...
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Main Authors | , , , |
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Format | Patent |
Language | English French German |
Published |
25.11.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided. |
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Bibliography: | Application Number: EP20180190196 |