CHROMATOGRAPHIC METHOD FOR ISOLATING AND PURIFYING HIGH-PURITY RECOMBINED HUMAN SERUM ALBUMIN

Disclosed is a chromatographic method for isolating and purifying high-purity recombined human serum albumin, comprising: conducting cation-exchange chromatography on recombined human serum albumin, and adding alcohol to a buffer solution to remove endotoxin, so as to obtain a primary product I; und...

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Bibliographic Details
Main Authors YANG, Daichang, OU, Jiquan, SHI, Bo, SHI, Qianni, LIU, Jingru
Format Patent
LanguageEnglish
French
German
Published 18.03.2020
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:Disclosed is a chromatographic method for isolating and purifying high-purity recombined human serum albumin, comprising: conducting cation-exchange chromatography on recombined human serum albumin, and adding alcohol to a buffer solution to remove endotoxin, so as to obtain a primary product I; under binding conditions , conducting anion/hydrophobic composite resin exchange chromatography on the primary product I to obtain an intermediate product II; conducting hydrophobic chromatography on the intermediate product II to obtain the target product, the purified high-purity recombined human serum albumin. The purity of the recombined human serum albumin obtained by isolating and purifying with the chromatographic method of the present invention is greater than 99.9999%, and the content of the endotoxin meets the criteria stipulated by Chinese pharmacopoeia.
Bibliography:Application Number: EP20180174112