RECOMBINANT MAMMALIAN CELLS AND METHOD FOR PRODUCING SUBSTANCE OF INTEREST

There are provided a recombinant cell that enables efficient production of protein, a method for producing the same, and a method for producing protein by using the cell. The cell is a mammalian cell having integrated therein 3 or more expression units containing an exogenous gene of interest, where...

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Bibliographic Details
Main Authors YAMANA, Yoshimasa, KONDO, Masako, KAMIHIRA, Masamichi
Format Patent
LanguageEnglish
French
German
Published 08.12.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:There are provided a recombinant cell that enables efficient production of protein, a method for producing the same, and a method for producing protein by using the cell. The cell is a mammalian cell having integrated therein 3 or more expression units containing an exogenous gene of interest, wherein gap elements are provided among multiple expression units, wherein the gap elements are designed to have chain lengths of 0.5 kbp or more, and the gap elements are mutually different sequences. Such a cell can produce recombinant protein stably for a long period and, especially, can show a strong positive linear correlation between the number of genes of interest introduced therein and the expression levels.
Bibliography:Application Number: EP20180164547