SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES

• Main Author: CANTOR, Charles R
• Format: Patent
• Language: English; French; German
• Published: 11.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a collection of solid supports comprising single-stranded nucleic acid species, (a) wherein each solid support in the collection comprises more than one single-stranded nucleic acid species covalently linked to the solid support, wherein each single-stranded nucleic acid species linked to a common solid support comprises an identifier sequence and a probe sequence, wherein the single-stranded nucleic acid species of each solid support in the collection further comprises a primer sequence, and (b) wherein all the single-stranded nucleic acid species linked to the common solid support share a common identifier sequence, and wherein the identifier sequence from the more than one single-stranded nucleic acid species linked to the common solid support in the collection is different from the identifier sequences of the single-stranded nucleic acid species linked to the other solid supports in the collection, whereby each solid support in the collection is associated with a unique identifier sequence each differing from all other identifier sequences by one or more nucleotides, when the identifier sequences are aligned with one another; and (c) wherein the probe sequence of each of the more than one single-stranded nucleic acid species linked to the solid support allows the more than one single-stranded nucleic acid to hybridize to a subsequence in a sample nucleic acid, and (d) wherein the probe sequences of the more than one single-stranded nucleic acid species on each solid support comprise a plurality of different probe sequence species, wherein each probe sequence species differs from all other probe sequence species by one or more nucleotides, when the probe sequence species are aligned with one another.