METHOD FOR OBTAINING A GLYCOPROTEIN WITH AN INCREASED PERCENTAGE OF AFUCOSYLATED GLYCANS

The present invention relates to a method for obtaining a glycoprotein with a glycoform composition comprising an increased percentage of afucosylated glycans relative to an identical method performed without arginine which comprises: (i) culturing cells expressing said glycoprotein under conditions...

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Bibliographic Details
Main Authors REAL FERNÁNDEZ, Rebeca, SÁNCHEZ DE MELO, Iván, SZEKRENYES, Akos, BES, Cedric
Format Patent
LanguageEnglish
French
German
Published 27.06.2018
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a method for obtaining a glycoprotein with a glycoform composition comprising an increased percentage of afucosylated glycans relative to an identical method performed without arginine which comprises: (i) culturing cells expressing said glycoprotein under conditions adequate for the expression of said glycoprotein in a cell culture medium comprising arginine in a concentration range of between about 1.5 and about 10 g/L; and (ii) recovering and purifying said glycoprotein.
Bibliography:Application Number: EP20160382631