SCREENING KIT AND METHODS

• Main Authors: BOCCHI, Massimo, ROCCHI, Laura, BISCARINI, Dario, FAENZA, Andrea, PECORARI, Nicola
• Format: Patent
• Language: English; French; German
• Published: 07.06.2023
• Subjects: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; TESTING; TRANSPORTING

The present invention relates to a method for the large-scale high-content analysis of biological samples, implemented in a reversed open microwell system which comprises an array of open microwells, at least one microchannel, at least one input port for reagents and/or for one or more biological samples and at least one output port for them, said input and output ports being in microfluidic communication with one or more of said microchannels, wherein said microchannel has a cross-section area of micrometric dimensions and provides fluid to said microwells, wherein said reversed open microwell system is inserted in an automated management system which comprises the following features: an incubator at controlled temperature, humidity and CO 2 , fluid dispensing system, phase-contrast and fluorescence image acquisition. The present invention further relates to a kit for introducing fluids in a microfluidic device and a system for discharging one or more fluids in a microfluidic device.