COMPOUNDS AND METHODS FOR DETECTION OF ENZYMES THAT REMOVE SUCCINYL OR METHYL SUCCINYL GROUPS FROM EPSILON AMINO LYSINE MOIETIES

• Main Authors: ZHANG, Zhongda, KISIELEWSKI, Anne, PATTON, Wayne Forrest, DALE, Elizabeth, HOWITZ, Konrad T
• Format: Patent
• Language: English; French; German
• Published: 04.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Provided is a compound that comprises the structure: (I) where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an µ-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an µ-amino of a lysine.