METHOD FOR THE DETECTION OF LEGIONELLA SPP. STRAINS IN ENVIRONMENTAL SAMPLES BASED ON LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP), DETECTION REAGENT AND SET OF PRIMERS

• Main Authors: VERDOY BERASTEGUI, Dolores, OLABARRIA DE PABLO, Garbiñe
• Format: Patent
• Language: English; French; German
• Published: 16.01.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The present invention relates to a method based on loop-mediated isothermal amplification (LAMP) designed to amplify specifically sequences of Legionella's 16S ribosomal RNA (rRNA) gene using a set of specially designed LAMP primers, to a detection reagent for specifically detecting sequences of Legionella's 16S rRNA gene, to a set of LAMP primers to amplify specific regions of Legionella's 16S rRNA gene, to a device designed for dosing the reagents and preparing the sample for analysis, to a support designed to house the device and to house: magnetic means, heating means, and optionally a UV-source, and to a system for dosing and preparing the sample for analysis.