ERROR-FREE SEQUENCING OF DNA
The invention relates to a novel method of error-free sequencing of DNA. Further, the present invention provides for a four-part oligonucleotide, comprising a fixed sequence, a randomized sequence, a restriction nuclease recognition site and/or restriction site, and a primer binding site. The invent...
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Main Authors | , , , |
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Format | Patent |
Language | English French German |
Published |
03.04.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to a novel method of error-free sequencing of DNA. Further, the present invention provides for a four-part oligonucleotide, comprising a fixed sequence, a randomized sequence, a restriction nuclease recognition site and/or restriction site, and a primer binding site. The invention also relates to the use of the sequenced DNA fragments obtained by the methods of the invention in methods for DNA sequence analysis, generation of cell lineage trees or assessment of copy numbers. |
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Bibliography: | Application Number: EP20150706700 |