PRODUCTION OF FATTY ACID ESTERS

• Main Authors: HOGSETT, DAVID A, RIBBLE, WENDY K, SPINDLER, EILEEN C, GRAMMANN, KATRIN, LIAO, HANS H, WARNECKE LIPSCOMB, TANYA, WOLTER, JAN, POOR, CATHERINE B, REINECKE, LIV, PRATHER, BRITTANY L, EVANS, RONALD J, SCHAFFER, STEFFEN
• Format: Patent
• Language: English; French; German
• Published: 06.04.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a microbial cell for producing at least one fatty acid ester, wherein the cell is genetically modified to comprise - at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by means of increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid comprises at least 5 carbon atoms; and - a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalysing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.