EARLY DETECTION AND ELIMINATION OF NON GERM-LINE EVENTS IN THE SOYBEAN TRANSFORMATION PROCESS

• Main Authors: PAREDDY, DAYAKAR, SAMUEL, JAYAKUMAR P, CHENNAREDDY, SIVARAMA R, MINNICKS, TATYANA, BOWLING, ANDY, SMITH, KELLEY A, YAU, KERRM Y, WHITTECK, JOHN
• Format: Patent
• Language: English; French; German
• Published: 20.01.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants which are comprised of a combination of soybean non-germline transformants and soybean germline transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are detected and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the transformation process.