METHODS AND COMPOSITIONS FOR PCR USING BLOCKED AND UNIVERSAL PRIMERS

Provided herein are methods and compositions for performing PCR with primers with blocked 3′-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Als...

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Bibliographic Details
Main Authors GUEGLER, Karl, SPIER, Eugene
Format Patent
LanguageEnglish
French
German
Published 15.02.2017
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Summary:Provided herein are methods and compositions for performing PCR with primers with blocked 3′-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations.
Bibliography:Application Number: EP20140737615