METHOD FOR PRODUCING, ISOLATING AND PURIFYING RECOMBINANT HUMAN ANTITRYPTASE (OSRAAT) FROM RICE SEEDS

Provided in the present invention are a rice genetic codon-optimized OsrAAT gene, related vector and a method for expression of OsrAAT in transgenic rice seeds by using the vector and a method for isolating and purifying OsrAAT. A transgenic rice seed expressing OsrAAT is prepared by the endosperm-s...

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Bibliographic Details
Main Authors SHI, QIANNI, YANG, DAICHANG, ZHANG, LIPING
Format Patent
LanguageEnglish
French
German
Published 16.09.2015
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
ORGANIC CHEMISTRY
PEPTIDES
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:Provided in the present invention are a rice genetic codon-optimized OsrAAT gene, related vector and a method for expression of OsrAAT in transgenic rice seeds by using the vector and a method for isolating and purifying OsrAAT. A transgenic rice seed expressing OsrAAT is prepared by the endosperm-specific expression vector. The purity of OsrAAT isolated and purified from transgenic rice grains reached 97% with HPLC grade and the yield of OsrAAT was up to 18.89 ± 3.19% of total soluble proteins, thus corresponding that 0.366 g of OsrAAT per kilogram can be produced from brown rice.
Bibliography:Application Number: EP20130853496