ENHANCED ACYLTRANSFERASE POLYNUCLEOTIDES, POLYPEPTIDES, AND METHODS OF USE

The invention provides modified DGAT1 proteins that are modified in the N-terminal region upstream of the acyl-Co A binding site. The modified DGAT proteins show enhanced activity, without reduced protein accumulation when expressed in cells. The modified DGAT1 proteins of the invention can be expre...

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Main Authors ROBERTS, NICHOLAS JOHN, WINICHAYAKUL, SOMRUTAI, SCOTT, RICHARD WILLIAM, CURRAN, AMY CHRISTINA, ROLDAN, MARISSA
Format Patent
LanguageEnglish
French
German
Published 20.04.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides modified DGAT1 proteins that are modified in the N-terminal region upstream of the acyl-Co A binding site. The modified DGAT proteins show enhanced activity, without reduced protein accumulation when expressed in cells. The modified DGAT1 proteins of the invention can be expressed in cells to increase cellular lipid accumulation and/or modify the cellular lipid profile. The invention also provides polynucleotides encoding the modified DGAT1 proteins, cells and compositions comprising the polynucleotides or modified DGAT proteins, and methods using the modified DGAT1 proteins to produce oil.
Bibliography:Application Number: EP20130852053