NOVEL FAR RED FLUORESCENT PROTEIN
The present invention relates to a nucleic acid molecule encoding a polypeptide having a fluorescence emission activity at a wavelength of at least 630 nm, wherein said nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule encoding a polypeptide having the amino...
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Main Authors | , , , , |
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Format | Patent |
Language | English French German |
Published |
17.06.2015
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention relates to a nucleic acid molecule encoding a polypeptide having a fluorescence emission activity at a wavelength of at least 630 nm, wherein said nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule encoding a polypeptide having the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule encoding a polypeptide having the amino acid sequence of SEQ ID NO: 1, with the exception that in the polypeptide of SEQ ID NO: 1 (b-i) glutamic acid at the position corresponding to position 16 is replaced by glycine; (b-ii) asparagine at the position corresponding to position 125 is replaced by aspartic acid; and/or (b-iii) lysine at the position corresponding to position 185 is replaced by asparagine; (c) a nucleic acid molecule encoding a polypeptide having at least 94% sequence identity to the polypeptide encoded by the nucleic acid molecule of (a) or (b); (d) a nucleic acid molecule having the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule having the nucleotide sequence of SEQ ID NO: 2, with the exception that said nucleic acid molecule has (e-i) at the positions corresponding to positions 48 to 50 of SEQ ID NO: 2 a nucleotide triplet selected from the group consisting of GGT, GGC, GGA and GGG; or (e-ii) at the positions corresponding to positions 375 to 377 of SEQ ID NO: 2 a nucleotide triplet selected from the group consisting of GAT and GAC; and/or (e-iii) at the positions corresponding to positions 555 to 557 of SEQ ID NO: 2 a nucleotide triplet selected from the group consisting of AAT and AAC; (f) a nucleic acid molecule having the nucleotide sequence of (d), (e) or (f), wherein each thymine is replaced by uracile; and (g) a nucleic acid molecule degenerate with respect to the nucleic acid molecule of (d) or (e). The present invention further relates to a vector comprising the nucleic acid molecule of the invention and a non-human host transformed with the vector of the invention. The present invention is further directed to a polypeptide having a fluorescence emission activity at a wavelength of at least 630 nm, a method of producing said polypeptide as well as fusion proteins comprising the polypeptide of the invention. Furthermore, the present invention relates to a diagnostic composition as well as a kit and to methods of detecting the expression of a gene of interest, detecting the activity of a promoter of interest, detecting the presence of a protein of interest and detecting the localization of a polypeptide or a fusion protein of the invention in a cell or tissue. |
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Bibliography: | Application Number: EP20130747409 |