MODIFIED OLIGONUCLEOTIDES COMPRISING THIOL FUNCTIONS AND THE USE OF SAME FOR THE DETECTION OF NUCLEIC ACIDS

The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, prefe...

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Bibliographic Details
Main Authors LEREAU, MYRIAM, VASSEUR, JEAN-JACQUES, MAYEN, JULIE, MORVAN, FRANÇOIS, MEYER, ALBERT, CHAIX, CAROLE, CANTALOUBE, JEAN-FRANÇOIS, FARRE, CAROLE, FOURNIER-WIRTH, CHANTAL
Format Patent
LanguageEnglish
French
German
Published 15.04.2015
Subjects
CHEMISTRY
DERIVATIVES THEREOF
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PHYSICS
SUGARS
TESTING
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Summary:The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, preferably maleimide or acrylamide functions. The invention also relates to a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridization between a modified oligonucleotide and a target nucleic acid amplified from the biological sample. The invention relates more particularly to a method for detecting, genotyping or sequencing a pathogenic organism, preferably a virus.
Bibliography:Application Number: EP20130716756