METHOD TO IDENTIFY LIGANDS FOR SIGMA-1 RECEPTORS

An assay for identifying ligands of the à 1 receptor based on a fusion protein comprising the à 1 receptor flanked by two fluorophores, so that said fluorophores are capable of producing constitutive FRET when no ligand is bound to the à 1 receptor. The fusion protein of the invention also allows th...

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Bibliographic Details
Main Authors CIRUELA ALFÉREZ, Francisco, BURGUEÑO-HURTADO, Javier, VELA HERNÁNDEZ, José Miguel
Format Patent
LanguageEnglish
French
German
Published 07.12.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:An assay for identifying ligands of the à 1 receptor based on a fusion protein comprising the à 1 receptor flanked by two fluorophores, so that said fluorophores are capable of producing constitutive FRET when no ligand is bound to the à 1 receptor. The fusion protein of the invention also allows the simultaneous determination whether the newly determined ligand is an agonist or an antagonist. Said fusion protein has been expressed in a cell, giving rise to a new cellular model useful for identifying à 1 receptor ligands, and additionally for discriminating between agonists and antagonists.
Bibliography:Application Number: EP20130700636