METHOD FOR DETECTING FUNGI, REACTION SOLUTION FOR PCR, AND CARRIER FOR DETECTING FUNGI

• Main Authors: KOKAJI, MAMI, TAKEHARU, HITOSHI, ISSHIKI, ATSUNORI, TANABE, SUGURU
• Format: Patent
• Language: English; French; German
• Published: 16.04.2014
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention enables highly accurate and specific detection of various types of fungi over a wide range in an inspection for confirming the presence or absence of fungi. Provided is a method for detecting fungi which comprises a step for amplifying DNA fragments containing target regions of fungal DNA and confirming the presence or absence of an amplified product, wherein the ITS region and the ²-tubulin gene are used as the target regions. Also provided is a reaction solution for PCR to be used for amplifying the target regions, wherein the concentration ratio between a primer set for amplifying ²-tubulin gene and a primer set for amplifying ITS region is within the range of 1:0.9 to 1:0.1.