Method for the biotechnological manufacture of dihydrochalcones

• Main Authors: Gall, Mechthild, Scheper, Prof. Dr. rer. nat. Tomas, Ley, Dr. Jakob Peter, Gross, Egon, Peters, Christin, Beutel, Dr. rer. nat. Sascha, Krammer, Dr. Gerhard, Hilmer, Dr. Jens-Michael, Thomsen, Maren, Jonczyk, Patrick, Bornscheuer, Prof. Dr. rer. nat. Uwe
• Format: Patent
• Language: English; French; German
• Published: 07.12.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

Producing dihydrochalcone, preferably phloretin, using a transgenic microorganism comprises: (iv) providing a transgenic microorganism comprising a nucleic acid segment (a) comprising a bacterial chalcone isomerase encoding gene and/or nucleic acid segment (a1) comprising a plant chalcone isomerase encoding gene, and a nucleic acid segment (b) comprising a bacterial enoate reductase encoding gene and (v) adding one or more flavanones, preferably naringin, and/or their precursors or derivatives, to the transgenic microorganism and culturing the transgenic microorganism. Producing dihydrochalcone, preferably phloretin, using a transgenic microorganism comprises: (iv) providing a transgenic microorganism comprising a nucleic acid segment (a) comprising a bacterial chalcone isomerase encoding gene and/or nucleic acid segment (a1) comprising a plant chalcone isomerase encoding gene, and a nucleic acid segment (b) comprising a bacterial enoate reductase encoding gene, (v) adding one or more flavanones, preferably naringin, and/or their precursors or derivatives, to the transgenic microorganism and culturing the transgenic microorganism and (vi) optionally isolating and purifying the dihydrochalcone. Independent claims are included for: (1) the transgenic microorganism comprising (a), (a1) and (b); (2) vector, preferably plasmid, comprising the transgenic microorganism containing (a), (a1) and (B); and (3) a host cell comprising one or more identical or different vectors.